Afg1 (ATPase Family Gene 1) is a mitochondrial protein associated with the inner mitochondrial membrane, which acts to maintain mitochondrial proteostasis. While Afg1 is a relatively understudied protein, a few key characteristics have been determined such as it having general refoldase activity, playing a role in the TIM22 import pathway, and playing a role in oxidative stress tolerance. As declining mitochondrial function is a characteristic of many neurodegenerative disorders, studying Afg1 has applications in improving human health, as Afg1 has been shown to be essential in maintaining mitochondrial and overall cellular health. While Afg1 is a yeast protein, there is a human/worm homologue, LACE-1, which has shown complementation to Afg1, indicating conservation among species. My research continues to explore the role Afg1 plays in the mitochondria. My current work includes attempting protein purification and structural determination, and identification of interacting partners.