Seminar
Time:
Biomedical Engineering Seminar: Dr. Alex Walsh
Date:
12:00 pm –
1:00 pm
Scott Engineering Center Room: 237
Contact:
Kayla Person, (402) 472-7079, kperson4@unl.edu
Dr. Walsh’s talk entitled “Imaging Cell Hetergeneity with Quantitative Autofluorescence Lifetime Microscopy” will be delivered in a hybrid format, so that attendees can attend in person (SEC 237) and also via Zoom.
https://unl.zoom.us/j/98036692041?pwd=ZkJBb1Vra3JPaW5Xa0RwRmhPWlltQT09
Meeting ID: 980 3669 2041
Passcode: COEBME
Description: Our understanding of how cellular heterogeneity contributes to disease progression and patient response to cell-based therapies is limited by our tools that can evaluate cells at the single-cell level. High-resolution optical microscopy allows non-destructive imaging of cells and tissues and can be combined with image segmentation for cell-level analysis. Two endogenous molecules, reduced nicotinamide adenine dinucleotide (NADH) and flavin adenine dinucleotide (FAD), exhibit fluorescence and enable label-free imaging of cells. Due to the function of NADH and FAD within metabolic reactions, label-free fluorescence imaging provides functional information of cellular metabolism. For cancer cells, autofluorescence lifetime microscopy provides over 90% accuracy for the prediction of metabolic pathway dependence on glycolysis or oxidative phosphorylation. Fluorescence lifetime imaging and single-cell image analysis of T cells allows classification of T cell activation state from autofluorescence imaging features with 97–99% accuracy. Label-free autofluorescence imaging of cell heterogeneity may be useful for imaging dynamic cell behaviors and quality control of biomanufactured cells.
Biography: Dr. Walsh completed her Ph.D. at Vanderbilt University where she developed an autofluorescence lifetime-based assay for measuring cancer therapy responses of patient-derived organoids. As a post-doc at the Air Force Research Lab, Dr. Walsh used optical techniques to investigate infrared-light activation and inhibition of action potential propagation in neurons. Currently, Dr. Walsh is an Assistant Professor in the Biomedical Engineering Department at Texas A&M University, where her lab group develops tools to detect and quantify cellular heterogeneity, identifies functional, label-free biomarkers of disease progression and drug response, and integrates biomarkers and microscopy tools clinically for improved health outcomes.
https://unl.zoom.us/j/98036692041?pwd=ZkJBb1Vra3JPaW5Xa0RwRmhPWlltQT09
Meeting ID: 980 3669 2041
Passcode: COEBME
Description: Our understanding of how cellular heterogeneity contributes to disease progression and patient response to cell-based therapies is limited by our tools that can evaluate cells at the single-cell level. High-resolution optical microscopy allows non-destructive imaging of cells and tissues and can be combined with image segmentation for cell-level analysis. Two endogenous molecules, reduced nicotinamide adenine dinucleotide (NADH) and flavin adenine dinucleotide (FAD), exhibit fluorescence and enable label-free imaging of cells. Due to the function of NADH and FAD within metabolic reactions, label-free fluorescence imaging provides functional information of cellular metabolism. For cancer cells, autofluorescence lifetime microscopy provides over 90% accuracy for the prediction of metabolic pathway dependence on glycolysis or oxidative phosphorylation. Fluorescence lifetime imaging and single-cell image analysis of T cells allows classification of T cell activation state from autofluorescence imaging features with 97–99% accuracy. Label-free autofluorescence imaging of cell heterogeneity may be useful for imaging dynamic cell behaviors and quality control of biomanufactured cells.
Biography: Dr. Walsh completed her Ph.D. at Vanderbilt University where she developed an autofluorescence lifetime-based assay for measuring cancer therapy responses of patient-derived organoids. As a post-doc at the Air Force Research Lab, Dr. Walsh used optical techniques to investigate infrared-light activation and inhibition of action potential propagation in neurons. Currently, Dr. Walsh is an Assistant Professor in the Biomedical Engineering Department at Texas A&M University, where her lab group develops tools to detect and quantify cellular heterogeneity, identifies functional, label-free biomarkers of disease progression and drug response, and integrates biomarkers and microscopy tools clinically for improved health outcomes.