Pallid Sturgeon (Scaphirhynchus albus) are centenarian, potamodromous, rheophiles that historically occupied the Missouri River and Mississippi River basins. Listed on the U.S. Endangered Species Act in 1990, population declines are attributed to habitat fragmentation and degradation, as well as overharvest, and hybridization. A knowledge gap exists regarding the extent to which tributaries facilitate key life stages for Pallid Sturgeon. This study evaluated the capacity of acoustic telemetry to monitor the movements of Pallid Sturgeon in a shallow, braided tributary to the Missouri River. The specific objectives were to (1) evaluate the environmental variables influencing the performance of acoustic telemetry, and to (2) quantify movement, residency, and seasonal space use of adult and sub-adult Pallid Sturgeon in the lower Platte River, Nebraska. Rigorous range testing protocols were employed, revealing relatively low detection ranges (<200 m) compared to other rivers throughout the region. Long-term reference tags revealed high variability in detection range, influenced primarily by line-of-sight, discharge, and water depth. Viewshed analyses were also employed to quantify detection range and may be a cost-effective alternative to formal range testing in systems where line-of-sight limits detection. A total of 80 Pallid Sturgeon were detected through active and passive telemetry, including sub-adults, reproductive adults, and non-reproductive adults. Space use models were created for 72 adults and 6 sub-adults. Pallid Sturgeon movements predominantly occurred at night, with a higher degree of residency in the Platte River compared to the Missouri River. Further, adult Pallid Sturgeon exhibited greater space use areas compared to subadults. Distinct migration strategies were documented, including one-step and two-step migrations, with overwintering occurring throughout the lower Platte River basin. Results confirmed that, despite low performance of acoustic telemetry in shallow, sand-braided rivers, species-specific designs can yield valuable movement information. The study emphasized that the lower Platte River basin provides important resources for the survival and persistence of multiple life-stages of Pallid Sturgeon.